mouse renal carcinoma cell line renca  (Procell Inc)

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Therapeutic effects and underlying mechanism of poly (L-glutamic acid)- g -methoxy poly (ethylene glycol)/combretastatin A4/BLZ945 nanoparticles on Renca renal carcinoma

doi: 10.3389/fbioe.2024.1336692

Figure Lengend Snippet: Impacts of CB-NPs on the Renca renal carcinoma cell line. (A) The cytotoxicity of CB-NPs on the Renca cell line was detected using the CCK-8 method ( n = 3). (B) The Transwell cell migration and invasion experiments were performed on the Renca cell line, and the results were observed under a ×200 magnification ( n = 3). (C) Significant difference observed in Transwell cell migration between PBS and CB-NPs groups, * p < 0.05. (D) Significant difference observed in Transwell cell invasion between PBS and CB-NPs groups, ** p < 0.01.

Article Snippet: The mouse renal carcinoma (Renca) cell lines were purchased from Procell under the conditions of RPMI-1640 (Seven) + 10% FBS (ABCELL) + 1% P/S (Seven) and incubated at 37°C and 5% CO 2 .

Techniques: CCK-8 Assay, Migration

Journal: Oncology Letters

Article Title: Nucleobindin-2 enhances the epithelial-mesenchymal transition in renal cell carcinoma

doi: 10.3892/ol.2020.11526

Figure Lengend Snippet: Suppression of NUCB-2 in vivo inhibits tumor formation in a renal cell carcinoma mouse model. (A) Relative mRNA levels of NUCB-2 normalized to those of GAPDH in shRNA or control cell lines. *P<0.05. (B) Protein expression levels of NUCB-2 in the shRNA and control cell lines. (C) Densitometry and statistical analysis of the western blots in the right panel. Relative protein levels of NUCB-2 normalized to those of GAPDH in samples. *P<0.05. (D) BALB/c mice were subcutaneously injected with Renca renal carcinoma cells in the rear flanks, and tumor size was measured every 2 days for the following 3 weeks. Growth of Renca cell tumors in a mouse model following the injection of an shRNA targeting NUCB-2 or a control injection. (E) In vivo tumors from mice implanted with the two different cell lines. (F) Relative protein expression levels of NUCB-2 normalized to those of GAPDH in shRNA or control cell lines at the end of the in vivo experiments. (G) Protein expression of NUCB-2 in the shRNA or control cell lines at the end of the in vivo experiment. *P<0.05. NUCB-2, nucleobindin 2; sh, short hairpin.

Article Snippet: The mouse renal carcinoma cell line Renca was purchased from the American Type Culture Collection.

Techniques: In Vivo, shRNA, Control, Expressing, Western Blot, Injection